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Stackpole et al

Bound C1q or deposited C3, C4 or C5 was detected using specific primary antibodies from rabbit (all from Dako/Agilent, A0135, A0062, A0065, A0056) (diluted 1:5000 in PBS/T/S) and a secondary HRP-conjugated anti-rabbit IgG antibody from donkey (Cytiva Life Sciences, NA935) (diluted 1:10.000 in PBS/T/S)

FIGURE 3 FIGURE 4 FIGURE 5 It is known that NPM interacts with ARF in the nucleolus, and after DNA damage, both can translocate to the nucleoplasm and to cause disruption of p53-HDM2 interaction (Kurki et al., 2004b), which allow p53 stabilization and activation by, for example, phosphorylation in Ser15 (Loughery et al., 2014)

All data were acquired in profile mode using positive polarity and peptide match was set to off, and isotope exclusion was on

The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity